nivalis as it switches to a non-growth state, whereas C. reinhardtii does not have this response. These differences in protein abundance have given greater understanding of the mechanism by which salt stress promotes fatty acid accumulation in the un-sequenced microalga C.
Data are available via ProteomeXchange with identifier PXD018148. Decreases in these enzymes have been shown to lead to increased TAG production as fatty acid biosynthesis is favoured. nivalis there were decreases in the abundance of UDP-sulfoquinovose (− 1.77-fold change), which is involved in sulfoquinovosyl diacylglycerol metabolism, and in citrate synthase (− 2.7-fold change), also involved in the TCA cycle. reinhardtii remained unaffected this enzyme is involved in acetyl CoA production and has been linked to TAG accumulation in microalgae. nivalis (1.1-fold change), whilst levels in C.
In terms of lipid synthesis, salt stress induced an increase in dihydrolipoyl dehydrogenase in C. There were abundance differences in proteins associated with stress, photosynthesis, carbohydrate and lipid metabolism proteins. De novo sequencing and homology matching was used in conjunction with iTRAQ-based quantitative analysis to identify and relatively quantify proteomic alterations in cells exposed to salt stress. Photosynthesis and respiration rates are reduced in C. nivalis fatty acid profiles showed that salt stress improved the biofuel qualities over time. In 0.2 M NaCl, C. nivalis accumulates carbohydrates up to 10.4% DCW at 80 h, and fatty acids up to 52.0% dry cell weight (DCW) over 12 days, however, C. reinhardtii does not show fatty acid accumulation over time, and shows limited carbohydrate accumulation up to 5.5% DCW. ResultsĮach strain was grown in conditions tailored to their growth requirements to encourage maximal fatty acid (as a proxy measure of lipid) production, with internal controls to allow comparison points. Here, we characterised physiological and proteomic changes between a low-starch C. reinhardtii strain and the snow alga Chlamydomonas nivalis, to reveal insights into their contrasting responses to salinity stress. Chlamydomonas reinhardtii is a model green alga strain for molecular studies its fully sequenced genome has enabled omic-based analyses that have been applied to better understand its metabolic responses to stress.
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